TFRC recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of K562 (A), Hela (B) whole cell lysates with TFRC recombinant monoclonal antibody (Cat # RAB02551).
Immunofluorescence
Immunofluorescent analysis of HeLa cells with TFRC recombinant monoclonal antibody (Cat # RAB02550). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human TFRC.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein of human TFRC.
Theoretical MW (kDa)
90
Reactivity
Human
Specificity
Recognizes endogenous levels of CD71 protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Immunoprecipitation (1:10-1:50)
Western Blot (1:500-1:1000)Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of K562 (A), Hela (B) whole cell lysates with TFRC recombinant monoclonal antibody (Cat # RAB02551).Immunocytochemistry
Immunofluorescence
Immunofluorescent analysis of HeLa cells with TFRC recombinant monoclonal antibody (Cat # RAB02550). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.Immunoprecipitation
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Gene Info — TFRC
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Interactome
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Pathway
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Disease
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