PTGS2 recombinant monoclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot analysis of rat brain (A) whole cell lysates with PGHS-2 recombinant monoclonal antibody (Cat # RAB02485).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human lung cancer formalin fixed paraffin embedded tissue section using PGHS-2 recombinant monoclonal antibody (Cat # RAB02485). . The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.78). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PTGS2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human COX2.
Theoretical MW (kDa)
70
Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of PGHS-2 protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of rat brain (A) whole cell lysates with PGHS-2 recombinant monoclonal antibody (Cat # RAB02485).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human lung cancer formalin fixed paraffin embedded tissue section using PGHS-2 recombinant monoclonal antibody (Cat # RAB02485). . The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.78). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Gene Info — PTGS2
Entrez GeneID
5743Protein Accession#
P35354Gene Name
PTGS2
Gene Alias
COX-2, COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2
Gene Description
prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
Omim ID
600262Gene Ontology
HyperlinkGene Summary
Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq
Other Designations
OTTHUMP00000033524|cyclooxygenase 2b|prostaglandin G/H synthase and cyclooxygenase|prostaglandin-endoperoxide synthase 2
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