TUBB recombinant monoclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of rat brain (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates with Beta-tubulin recombinant monoclonal antibody (Cat # RAB02475).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human tonsil formalin fixed paraffin embedded tissue section using Beta-tubulin recombinant monoclonal antibody (Cat # RAB02475). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.119). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human TUBB.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human beta Tubulin.
Theoretical MW (kDa)
55
Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of Beta-tubulin protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunoprecipitation(1:10-1:50)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of rat brain (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates with Beta-tubulin recombinant monoclonal antibody (Cat # RAB02475).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human tonsil formalin fixed paraffin embedded tissue section using Beta-tubulin recombinant monoclonal antibody (Cat # RAB02475). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.119). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.Immunoprecipitation
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Gene Info — TUBB
Entrez GeneID
203068Protein Accession#
P07437Gene Name
TUBB
Gene Alias
M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB1, TUBB5
Gene Description
tubulin, beta
Omim ID
191130Gene Ontology
HyperlinkGene Summary
O
Other Designations
OTTHUMP00000029069|beta 5-tubulin|beta Ib tubulin|beta-4 tubulin|tubulin beta polypeptide|tubulin beta-1 chain|tubulin beta-5 chain|tubulin, beta polypeptide
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Interactome
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Pathway
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Disease
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