CYCS recombinant monoclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of mouse brain (A), Jurkat (B), rat brain (C), C6 (D) whole cell lysates with Cytochrome c recombinant monoclonal antibody (Cat # RAB02443).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human tonsil formalin fixed paraffin embedded tissue section using Cytochrome c recombinant monoclonal antibody (Cat # RAB02443). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.114). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CYCS.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human Cytochrome C.
Theoretical MW (kDa)
14
Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of Cytochrome c protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunoprecipitation(1:10-1:50)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of mouse brain (A), Jurkat (B), rat brain (C), C6 (D) whole cell lysates with Cytochrome c recombinant monoclonal antibody (Cat # RAB02443).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human tonsil formalin fixed paraffin embedded tissue section using Cytochrome c recombinant monoclonal antibody (Cat # RAB02443). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.114). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.Immunoprecipitation
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Gene Info — CYCS
Entrez GeneID
54205Protein Accession#
P99999Gene Name
CYCS
Gene Alias
CYC, HCS
Gene Description
cytochrome c, somatic
Omim ID
123970Gene Ontology
HyperlinkGene Summary
This gene encodes cytochrome c, a component of the electron transport chain in mitochondria. The heme group of cytochrome c accepts electrons from the b-c1 complex and transfers electrons to the cytochrome oxidase complex. Cytochrome c is also involved in initiation of apoptosis. Upon release of cytochrome c to the cytoplasm, the protein binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Many cytochrome c pseudogenes exist, scattered throughout the human genome. [provided by RefSeq
Other Designations
cytochrome c
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Interactome
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Pathway
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Disease
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