Products

Western Blot (Cell lysate)

Western blot analysis of Hela (A) whole cell lysates with Caveolin 1 recombinant monoclonal antibody (Cat # RAB02423).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical analysis of human breast carcinoma formalin fixed paraffin embedded tissue section using Caveolin 1 recombinant monoclonal antibody (Cat # RAB02423). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.4). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunocytochemistry

Immunofluorescence

Immunofluorescent analysis of HeLa cells with Caveolin 1 recombinant monoclonal antibody (Cat # RAB02423). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Immunoprecipitation

Entrez GeneID

857

Protein Accession#

Q03135

Gene Name

CAV1

Gene Alias

BSCL3, CAV, CGL3, MSTP085, VIP21

Gene Description

caveolin 1, caveolae protein, 22kDa

Omim ID

601047

Gene Ontology

Hyperlink

Gene Summary

The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by a single transcript from this gene. [provided by RefSeq

Other Designations

OTTHUMP00000025031|caveolae protein, 22-kD|caveolin 1|caveolin 1 caveolae protein, 22kD|caveolin 1, alpha isoform|caveolin 1, beta isoform|caveolin-1 beta isoform protein|cell growth-inhibiting protein 32