BAX recombinant monoclonal antibody
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against recombinant BAX.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human Bax.
Theoretical MW (kDa)
22
Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of BAX protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunoprecipitation(1:10-1:50)
Western Blot (1:500-1:1000)Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of C6 (A), NIH3T3 (B), Hela (C) whole cell lysates with BAX recombinant monoclonal antibody (Cat # RAB02420).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human lung cancer formalin fixed paraffin embedded tissue section using BAX recombinant monoclonal antibody (Cat # RAB02420). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.75). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.Immunoprecipitation
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Gene Info — BAX
Entrez GeneID
581Protein Accession#
Q07812Gene Name
BAX
Gene Alias
BCL2L4
Gene Description
BCL2-associated X protein
Omim ID
600040Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq
Other Designations
apoptosis regulator BAX
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