Importin 9 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Jurkat (A), Hela (B) whole cell lysates with Importin 9 recombinant monoclonal antibody (Cat # RAB02411).
Immunofluorescence
Immunofluorescent analysis of A549 cells with Importin 9 recombinant monoclonal antibody (Cat # RAB02411). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against recombinant Importin 9.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human Importin 9.
Theoretical MW (kDa)
120
Reactivity
Human
Specificity
Recognizes endogenous levels of Importin 9 protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Immunoprecipitation (1:10-1:50)
Western Blot (1:500-1:1000)Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Jurkat (A), Hela (B) whole cell lysates with Importin 9 recombinant monoclonal antibody (Cat # RAB02411).Immunocytochemistry
Immunofluorescence
Immunofluorescent analysis of A549 cells with Importin 9 recombinant monoclonal antibody (Cat # RAB02411). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.Immunoprecipitation
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Gene Info — IPO9
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Interactome
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