PHD1 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts of Lane1:Hela whole cell lysate, Lane2:PC12 whole cell lysate, Lane3:NIH/3T3 whole cell lysate with PHD1 recombinant monoclonal antibody (Cat # RAB02396).
Immunoprecipitation-Western Blot
Immunoprecipitation analysis of 150 ug extracts of HeLa cells using 3ug PHD1 recombinant monoclonal antibody (Cat # RAB02396). Western blot was performed from the immunoprecipitate using PHD1 recombinant monoclonal antibody (Cat # RAB02396) at a dilution of 1:1000.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against recombinant PHD1.
Antibody Species
Rabbit
Theoretical MW (kDa)
44
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of PHD1 and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunoprecipitation (1:20-1:50)
Western Blot (1:500-1:2000)Storage Buffer
In PBS, pH 7.2 (0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts of Lane1:Hela whole cell lysate, Lane2:PC12 whole cell lysate, Lane3:NIH/3T3 whole cell lysate with PHD1 recombinant monoclonal antibody (Cat # RAB02396).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation-Western Blot
Immunoprecipitation analysis of 150 ug extracts of HeLa cells using 3ug PHD1 recombinant monoclonal antibody (Cat # RAB02396). Western blot was performed from the immunoprecipitate using PHD1 recombinant monoclonal antibody (Cat # RAB02396) at a dilution of 1:1000.Flow Cytometry
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Gene Info — EGLN2
Entrez GeneID
112398Protein Accession#
Q96KS0Gene Name
EGLN2
Gene Alias
DKFZp434E026, EIT6, HIFPH1, HPH-3, PHD1
Gene Description
egl nine homolog 2 (C. elegans)
Omim ID
606424Gene Ontology
HyperlinkGene Summary
The hypoxia inducible factor (HIF) is a transcriptional complex which is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this posttranslational modification. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
EGL nine (C.elegans) homolog 2|HIF prolyl hydroxylase 1|HIF-prolyl hydroxylase 1|estrogen-induced tag 6|hypoxia-inducible factor prolyl hydroxylase 1|prolyl hydroxylase domain-containing protein 1
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Interactome
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