EIF4A3 recombinant monoclonal antibody, clone R05-1B7
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More Files
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human EIF4A3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human EIF4A3.
Theoretical MW (kDa)
Calculated MW: 47 kD
Reactivity
Hamster, Human, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: Hela, Lane 2: CHO-K1 and Lane 3: C6 lysates with EIF4A3 recombinant monoclonal antibody, clone R05-1B7 (Cat # RAB02309).Immunohistochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — EIF4A3
Entrez GeneID
9775Protein Accession#
P38919Gene Name
EIF4A3
Gene Alias
DDX48, KIAA0111, MGC10862, NMP265, NUK-34, eIF4AIII, hNMP265
Gene Description
eukaryotic translation initiation factor 4A, isoform 3
Omim ID
608546Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family. [provided by RefSeq
Other Designations
DEAD (Asp-Glu-Ala-Asp) box polypeptide 48|eukaryotic initiation factor 4A-like NUK-34
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