GLS recombinant monoclonal antibody, clone R08-3D8
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human GLS.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human GLS.
Theoretical MW (kDa)
Calculated MW: 73 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence(1:50-1:200)
Immunoprecipitation(1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: K562, Lane 2: 3T3 and Lane 3: Hela lysates with GLS recombinant monoclonal antibody, clone R08-3D8 (Cat # RAB02251).Immunofluorescence
Immunoprecipitation
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Gene Info — GLS
Entrez GeneID
2744Protein Accession#
O94925Gene Name
GLS
Gene Alias
AAD20, DKFZp686O15119, FLJ10358, GLS1, KIAA0838
Gene Description
glutaminase
Omim ID
138280Gene Ontology
HyperlinkGene Summary
Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM
Other Designations
K-glutaminase|L-glutamine amidohydrolase|glutaminase C|glutaminase, phosphate-activated
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Interactome
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