H1-2 recombinant monoclonal antibody, clone R05-6F1
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human H1-2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human H1-2.
Theoretical MW (kDa)
Calculated MW: 21 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence(1:50-1:200)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: Hela, Lane 2: C6 and Lane 3: MCF-7 lysates with H1-2 recombinant monoclonal antibody, clone R05-6F1 (Cat # RAB02227).Immunohistochemistry
Immunofluorescence
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Gene Info — HIST1H1C
Entrez GeneID
3006Protein Accession#
P16403Gene Name
HIST1H1C
Gene Alias
H1.2, H1F2, MGC3992
Gene Description
histone cluster 1, H1c
Omim ID
142710Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq
Other Designations
H1 histone family, member 2|OTTHUMP00000017749|histone 1, H1c|histone H1d
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