MBD2 recombinant monoclonal antibody, clone R05-4G5
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: rat brain, Lane 2: C6 and Lane 3: Hela lysates with MBD2 recombinant monoclonal antibody, clone R05-4G5 (Cat # RAB02116).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with MBD2 recombinant monoclonal antibody, clone R05-4G5 (Cat # RAB02116). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human MBD2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human MBD2.
Theoretical MW (kDa)
Calculated MW: 43 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence(1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation(1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: rat brain, Lane 2: C6 and Lane 3: Hela lysates with MBD2 recombinant monoclonal antibody, clone R05-4G5 (Cat # RAB02116).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with MBD2 recombinant monoclonal antibody, clone R05-4G5 (Cat # RAB02116). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunofluorescence
Immunoprecipitation
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Gene Info — MBD2
Entrez GeneID
8932Protein Accession#
Q9UBB5Gene Name
MBD2
Gene Alias
DKFZp586O0821, DMTase, NY-CO-41
Gene Description
methyl-CpG binding domain protein 2
Omim ID
603547Gene Ontology
HyperlinkGene Summary
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. [provided by RefSeq
Other Designations
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Interactome
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Disease
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