MDH2 recombinant monoclonal antibody, clone R06-4E2
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More Files
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Western Blot
Western Blot analysis of Lane 1: K562, Lane 2: C6 and Lane 3: 3T3 lysates with MDH2 recombinant monoclonal antibody, clone R06-4E2 (Cat # RAB02113).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer with MDH2 recombinant monoclonal antibody, clone R06-4E2 (Cat # RAB020113). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human MDH2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human MDH2.
Theoretical MW (kDa)
Calculated MW: 36 kD
Reactivity
Human, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: K562, Lane 2: C6 and Lane 3: 3T3 lysates with MDH2 recombinant monoclonal antibody, clone R06-4E2 (Cat # RAB02113).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer with MDH2 recombinant monoclonal antibody, clone R06-4E2 (Cat # RAB020113). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. -
Gene Info — MDH2
Entrez GeneID
4191Protein Accession#
P40926Gene Name
MDH2
Gene Alias
M-MDH, MDH, MGC:3559, MOR1
Gene Description
malate dehydrogenase 2, NAD (mitochondrial)
Omim ID
154100Gene Ontology
HyperlinkGene Summary
Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. [provided by RefSeq
Other Designations
mitochondrial malate dehydrogenase
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Carbon fixation in photosynthetic organisms
- Citrate cycle (TCA cycle)
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