PFKL recombinant monoclonal antibody, clone R04-7A4
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More Files
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Western Blot
Western blot analysis of Lane 1: C6 and Lane 2: 3T3 lysates with PFKL recombinant monoclonal antibody, clone R04-7A4 (Cat # RAB02008).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PFKL.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human PFKL.
Theoretical MW (kDa)
Calculated MW: 85 kD
Reactivity
Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: C6 and Lane 2: 3T3 lysates with PFKL recombinant monoclonal antibody, clone R04-7A4 (Cat # RAB02008).Immunofluorescence
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Gene Info — PFKL
Entrez GeneID
5211Protein Accession#
P17858Gene Name
PFKL
Gene Alias
DKFZp686G1648, DKFZp686L2097, FLJ30173, FLJ40909, PFK-B
Gene Description
phosphofructokinase, liver
Omim ID
171860Gene Ontology
HyperlinkGene Summary
Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Alternate splicing results in two transcript variants, one of which is a candidate for nonsense-mediated decay (NMD). [provided by RefSeq
Other Designations
6-phosphofructokinase, liver type|liver phosphofructokinase|liver-type 1-phosphofructokinase|phosphofructo-1-kinase isozyme B|phosphofructokinase 1|phosphohexokinase
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Fructose and mannose metabolism
- Galactose metabolism
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