PUM1 recombinant monoclonal antibody, clone R04-3A6
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More Files
- More Functions
Western Blot
Western blot analysis of 3T3 lysates with PUM1 recombinant monoclonal antibody, clone R04-3A6 (Cat # RAB01971).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin/PFA-fixed paraffin-embedded sections) of human tonsil with PUM1 recombinant monoclonal antibody, clone R04-3A6 (Cat # RAB01971).
High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PUM1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human PUM1.
Theoretical MW (kDa)
Calculated MW: 126 k
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of 3T3 lysates with PUM1 recombinant monoclonal antibody, clone R04-3A6 (Cat # RAB01971).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin/PFA-fixed paraffin-embedded sections) of human tonsil with PUM1 recombinant monoclonal antibody, clone R04-3A6 (Cat # RAB01971).
High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunohistochemistry (Frozen sections)
Immunofluorescence
Immunoprecipitation
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Gene Info — PUM1
Entrez GeneID
9698Protein Accession#
Q14671Gene Name
PUM1
Gene Alias
HSPUM, KIAA0099, PUMH, PUMH1, PUML1
Gene Description
pumilio homolog 1 (Drosophila)
Omim ID
607204Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000003892|pumilio 1
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Interactome
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Disease
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