SCD recombinant monoclonal antibody, clone R04-2K1
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More Files
- More Functions
Western Blot
Western blot analysis of Hela lysates with SCD recombinant monoclonal antibody, clone R04-2K1 (Cat # RAB01924).
Immunocytochemistry
Immunocytochemical staining of Hela with SCD recombinant monoclonal antibody, clone R04-2K1 (Cat # RAB01924). (A) SCD (green) and (B) DAPI (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human SCD.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human SCD.
Theoretical MW (kDa)
Calculated MW: 42 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Hela lysates with SCD recombinant monoclonal antibody, clone R04-2K1 (Cat # RAB01924).Immunohistochemistry
Immunocytochemistry
Immunocytochemical staining of Hela with SCD recombinant monoclonal antibody, clone R04-2K1 (Cat # RAB01924). (A) SCD (green) and (B) DAPI (blue).Immunofluorescence
Immunoprecipitation
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Gene Info — SCD
Entrez GeneID
6319Protein Accession#
O00767Gene Name
SCD
Gene Alias
FADS5, MSTP008, SCD1
Gene Description
stearoyl-CoA desaturase (delta-9-desaturase)
Omim ID
604031Gene Ontology
HyperlinkGene Summary
Stearoyl-CoA desaturase (SCD; EC 1.14.99.5) is an iron-containing enzyme that catalyzes a rate-limiting step in the synthesis of unsaturated fatty acids. The principal product of SCD is oleic acid, which is formed by desaturation of stearic acid. The ratio of stearic acid to oleic acid has been implicated in the regulation of cell growth and differentiation through effects on cell membrane fluidity and signal transduction. Four SCD isoforms, Scd1 through Scd4, have been identified in mouse. In contrast, only 2 SCD isoforms, SCD1 and SCD5 (MIM 608370), have been identified in human. SCD1 shares about 85% amino acid identity with all 4 mouse SCD isoforms, as well as with rat Scd1 and Scd2. In contrast, SCD5 shares limited homology with the rodent SCDs and appears to be unique to primates (Zhang et al. (1999) [PubMed 10229681]; Wang et al., 2005 [PubMed 15907797]).[supplied by OMIM
Other Designations
OTTHUMP00000020279|acyl-CoA desaturase|delta-9-desaturase|fatty acid desaturase|predicted protein of HQ0998|stearoyl-CoA desaturase
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Interactome
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Disease
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