ACTL6A recombinant monoclonal antibody, clone R03-1G4
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More Files
- More Functions
Immunocytochemistry
Immunocytochemical staining of Jurkat with ACTL6A recombinant monoclonal antibody, clone R03-1G4 (Cat # RAB01768). (A) ACTL6A (green) and (B) DAPI (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human ACTL6A.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human ACTL6A.
Theoretical MW (kDa)
Calculated MW: 47 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence (1/50-1/200)
Immunoprecipitation (1/20)
Western Blot (1/500-1/1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunocytochemistry
Immunocytochemical staining of Jurkat with ACTL6A recombinant monoclonal antibody, clone R03-1G4 (Cat # RAB01768). (A) ACTL6A (green) and (B) DAPI (blue).Immunofluorescence
Immunoprecipitation
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Gene Info — ACTL6A
Entrez GeneID
86Protein Accession#
O96019Gene Name
ACTL6A
Gene Alias
ACTL6, ARPN-BETA, Arp4, BAF53A, INO80K, MGC5382
Gene Description
actin-like 6A
Omim ID
604958Gene Ontology
HyperlinkGene Summary
This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described. [provided by RefSeq
Other Designations
BAF complex 53 kDa subunit|BAF53|BRG1-associated factor|INO80 complex subunit K|actin-related protein 4|hArpN beta
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Interactome
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