DLD recombinant monoclonal antibody, clone R05-6D3
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More Files
- More Functions
Western Blot
Western blot analysis of Lipoamide Dehydrogenase in K562, rat Brain, C6, 3T3, Hela lysates using human Lipoamide Dehydrogenase recombinant monoclonal antibody, clone R05-6D3 (Cat # RAB01721).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer with Lipoamide Dehydrogenase recombinant monoclonal antibody, clone R05-6D3 (Cat # RAB01721). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human Lipoamide Dehydrogenase.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human Lipoamide Dehydrogenase
Theoretical MW (kDa)
Calculated MW: 54 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lipoamide Dehydrogenase in K562, rat Brain, C6, 3T3, Hela lysates using human Lipoamide Dehydrogenase recombinant monoclonal antibody, clone R05-6D3 (Cat # RAB01721).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer with Lipoamide Dehydrogenase recombinant monoclonal antibody, clone R05-6D3 (Cat # RAB01721). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunohistochemistry (Frozen sections)
Immunocytochemistry
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Gene Info — DLD
Entrez GeneID
1738Protein Accession#
P09622Gene Name
DLD
Gene Alias
DLDH, E3, GCSL, LAD, PHE3
Gene Description
dihydrolipoamide dehydrogenase
Gene Ontology
HyperlinkGene Summary
This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. [provided by RefSeq
Other Designations
E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex|diaphorase|dihydrolipoyl dehydrogenase|glycine cleavage system protein L|lipoamide dehydrogenase|lipoamide reductase|lipoyl dehydrogena
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Glycine
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