PFN1 recombinant monoclonal antibody, clone R06-6H7
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human Profilin 1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human Profilin 1
Theoretical MW (kDa)
Calculated MW: 15 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Profilin 1 in C6, 3T3, Hela lysates using human Profilin 1 recombinant monoclonal antibody, clone R06-6H7 (Cat # RAB01694).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) of Human tonsil with Profilin 1 recombinant monoclonal antibody, clone R06-6H7 (Cat # RAB01694).Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunocytochemical staining of A549 with Profilin 1 recombinant monoclonal antibody, clone R06-6H7 (Cat # RAB01694). (A) Profilin 1 (green) and (B) DAPI (blue).Immunoprecipitation
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Gene Info — PFN1
Entrez GeneID
5216Protein Accession#
P07737Gene Name
PFN1
Gene Alias
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Gene Description
profilin 1
Omim ID
176610Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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