GOT1 recombinant monoclonal antibody, clone R05-6C1
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More Files
- More Functions
Western Blot
Western blot analysis of Aspartate Aminotransferase in K562, rat Brain, 3T3, Hela lysates using human Aspartate Aminotransferase recombinant monoclonal antibody, clone R05-6C1 (Cat # RAB01584).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) of Human colon cancer tissue with Aspartate Aminotransferase recombinant monoclonal antibody, clone R05-6C1 (Cat # RAB01584).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human Aspartate Aminotransferase.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human Aspartate Aminotransferase
Theoretical MW (kDa)
Calculated MW: 46 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence(1:50-1:200)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Aspartate Aminotransferase in K562, rat Brain, 3T3, Hela lysates using human Aspartate Aminotransferase recombinant monoclonal antibody, clone R05-6C1 (Cat # RAB01584).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) of Human colon cancer tissue with Aspartate Aminotransferase recombinant monoclonal antibody, clone R05-6C1 (Cat # RAB01584).Immunohistochemistry (Frozen sections)
Immunocytochemistry
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Gene Info — GOT1
Entrez GeneID
2805Protein Accession#
P17174Gene Name
GOT1
Gene Alias
GIG18
Gene Description
glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
Omim ID
138180Gene Ontology
HyperlinkGene Summary
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq
Other Designations
OTTHUMP00000020254|aspartate aminotransferase 1|growth-inhibiting protein 18
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Interactome
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Pathway
- Alanine
- Arginine and proline metabolism
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Carbon fixation in photosynthetic organisms
- Cysteine and methionine metabolism
- Isoquinoline alkaloid biosynthesis
- Metabolic pathways
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