HMGB2 recombinant monoclonal antibody, clone R02-5I5
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More Files
- More Functions
Western Blot
Western blot analysis of HMGB2 in CHO-K1, Ramos lysates using HMGB2 antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded Human tonsil using HMGB2 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human HMGB2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human HMGB2.
Theoretical MW (kDa)
Calculated MW: 24 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence
Immunohistochemistry (Frozen sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4, (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of HMGB2 in CHO-K1, Ramos lysates using HMGB2 antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded Human tonsil using HMGB2 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — HMGB2
Entrez GeneID
3148Protein Accession#
P26583Gene Name
HMGB2
Gene Alias
HMG2
Gene Description
high-mobility group box 2
Omim ID
163906Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. [provided by RefSeq
Other Designations
high-mobility group (nonhistone chromosomal) protein 2
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Interactome
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Disease
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