NDUFS2 recombinant monoclonal antibody, clone R08-4D7
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More Files
- More Functions
Western Blot
Western blot analysis of NDUFS2 in Hela, HepG2, U2OS, U251, HL-60 lysates using NDUFS2 antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using NDUFS2 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human NDUFS2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human NDUFS2.
Theoretical MW (kDa)
Calculated MW: 53 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4, (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of NDUFS2 in Hela, HepG2, U2OS, U251, HL-60 lysates using NDUFS2 antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using NDUFS2 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunoprecipitation
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Gene Info — NDUFS2
Entrez GeneID
4720Protein Accession#
O75306Gene Name
NDUFS2
Gene Alias
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Gene Description
NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase)
Gene Ontology
HyperlinkGene Summary
Mitochondrial complex I (NADH-ubiquinone reductase; EC 1.6.5.3) is the first multimeric complex of the respiratory chain that catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mammalian mitochondrial complex I is an assembly of at least 43 different subunits. Seven of the subunits are encoded by the mitochondrial genome; the remainder are the products of nuclear genes. The iron-sulfur protein (IP) fraction of complex I is made up of 7 subunits, including NDUFS2. See NDUFS1 (MIM 157655).[supplied by OMIM
Other Designations
NADH dehydrogenase (ubiquinone) Fe-S protein 2|NADH-ubiquinone oxidoreductase Fe-S protein 2|NADH-ubiquinone oxidoreductase NDUFS2 subunit|OTTHUMP00000032241
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Interactome
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Pathway
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Disease
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