DLAT recombinant monoclonal antibody, clone R01-6I2
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human DLAT.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human DLAT.
Theoretical MW (kDa)
Calculated MW: 69 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4, (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Pyruvate Dehydrogenase E2 in CHO-K1, C6, rat Brain, Ramos lysates using Pyruvate Dehydrogenase E2 antibody.Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — DLAT
Entrez GeneID
1737Protein Accession#
P10515Gene Name
DLAT
Gene Alias
DLTA, PDC-E2, PDCE2
Gene Description
dihydrolipoamide S-acetyltransferase
Omim ID
608770Gene Ontology
HyperlinkGene Summary
The DLAT gene encodes dihydrolipoamide acetyltransferase (EC 2.3.1.12), the E2 subunit of the mammalian pyruvate dehydrogenase complex (PDC; EC 1.2.4.1) of the inner mitochondrial membrane. Patients with primary biliary cirrhosis (PBC; MIM 109720) show autoantibodies to DLAT.[supplied by OMIM
Other Designations
70 kDa mitochondrial autoantigen of primary biliary cirrhosis|E2 component of pyruvate dehydrogenase complex|M2 antigen complex 70 kDa subunit
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Glycolysis / Gluconeogenesis
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