PLK1 recombinant monoclonal antibody, clone R07-3E0
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More Files
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PLK1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human PLK1.
Theoretical MW (kDa)
Calculated MW: 68 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of CRCP in Hela, C6 lysates using CRCP antibody.Western Blot
Western blot analysis of PLK1 in Jurkat, C6 lysates using PLK1 antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using CRCP antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunocytochemistry
Immunocytochemistry analysis of PLK1 (green) in Hela using PLK1 antibody,and DAPI(blue).Immunofluorescence
Immunofluorescence
Immunoprecipitation
Immunoprecipitation
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Gene Info — PLK1
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Interactome
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Pathway
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Disease
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