PCNA recombinant monoclonal antibody, clone R09-6B3
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More Files
- More Functions
Western Blot
Western blot analysis of PCNA in K562, C6 lysates using PCNA antibody.
Immunocytochemistry
Immunocytochemistry analysis of PCNA(green) in Hela using PCNA antibody,and DAPI(blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PCNA3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human PCNA.
Theoretical MW (kDa)
Calculated MW: 29 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of PCNA in K562, C6 lysates using PCNA antibody.Immunocytochemistry
Immunocytochemistry analysis of PCNA(green) in Hela using PCNA antibody,and DAPI(blue).Immunofluorescence
Immunoprecipitation
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Gene Info — PCNA
Entrez GeneID
5111Protein Accession#
P12004Gene Name
PCNA
Gene Alias
MGC8367
Gene Description
proliferating cell nuclear antigen
Omim ID
176740Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq
Other Designations
DNA polymerase delta auxiliary protein|OTTHUMP00000030189|OTTHUMP00000030190|cyclin
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Interactome
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Pathway
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Disease
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