PIK3R1 recombinant monoclonal antibody, clone R03-3C7
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PIK3R1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against synthetic peptide corresponding to human PI 3 Kinase p85 alpha.
Theoretical MW (kDa)
Calculated MW: 84 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine pH 7.4 (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of PI3 Kinase p85 alpha in Jurkat, C6, CHO-K1 lysates using PI3 Kinase p85 alpha antibody.Immunocytochemistry
Immunocytochemistry analysis of PI3 Kinase p85 alpha (green) in MCF-7 using PI3 Kinase p85 alpha antibody,and DAPI(blue).Immunofluorescence
Immunoprecipitation
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Gene Info — PIK3R1
Entrez GeneID
5295Protein Accession#
P27986Gene Name
PIK3R1
Gene Alias
GRB1, p85, p85-ALPHA
Gene Description
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
Omim ID
171833Gene Ontology
HyperlinkGene Summary
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)|phosphatidylinositol 3-kinase, regulatory, 1|phosphatidylinositol 3-kinase-associated p-85 alpha|phosphoinositide-3-kinase, regulatory subunit 1 (p85 alpha)|phosphoinositide-3-ki
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Interactome
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Pathway
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Disease
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