PRKCB recombinant monoclonal antibody, clone R06-0C2
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More Files
- More Functions
Western Blot
Western blot analysis of PKC beta 2 in rat brain lysates using PKC beta 2 antibody.Observed band size:77kDa.
Immunocytochemistry
Immunocytochemistry analysis of PKC beta 2 (green) in K562 using PKC beta 2 antibody,and DAPI(blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PRKCB.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against synthesized peptide corresponding to human PKC beta 2.
Theoretical MW (kDa)
Calculated MW: 77 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot
Immunohistochemistry (Frozen sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine pH 7.4 (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of PKC beta 2 in rat brain lysates using PKC beta 2 antibody.Observed band size:77kDa.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunocytochemistry analysis of PKC beta 2 (green) in K562 using PKC beta 2 antibody,and DAPI(blue).Immunofluorescence
Immunoprecipitation
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Gene Info — PRKCB
Entrez GeneID
5579Protein Accession#
P05771Gene Name
PRKCB
Gene Alias
MGC41878, PKC-beta, PKCB, PRKCB1, PRKCB2
Gene Description
protein kinase C, beta
Omim ID
176970Gene Ontology
HyperlinkGene Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
protein kinase C, beta 1 polypeptide
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Interactome
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Pathway
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Disease
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