TRIM25 recombinant monoclonal antibody, clone R06-1H2
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More Files
- More Functions
Western Blot
Western blot analysis of TRIM25/EFP in K562, rat Brain, C6, Hela lysates using TRIM25 antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human TRIM25.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human TRIM25.
Theoretical MW (kDa)
Calculated MW: 71 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine pH 7.4 (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of TRIM25/EFP in K562, rat Brain, C6, Hela lysates using TRIM25 antibody.Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — TRIM25
Entrez GeneID
7706Protein Accession#
Q14258Gene Name
TRIM25
Gene Alias
EFP, RNF147, Z147, ZNF147
Gene Description
tripartite motif-containing 25
Omim ID
600453Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq
Other Designations
estrogen-responsive finger protein|tripartite motif protein TRIM25|zinc finger protein 147 (estrogen-responsive finger protein)|zinc finger protein-147
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Interactome
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Disease
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