CD22 recombinant monoclonal antibody, clone hL22 (Epratuzumab)
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More Files
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Flow Cytometry
Flow-cytometry were stained with an isotype control at a concentration of 1 ug/mL for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CD22.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against by the humanization of LL2, a murine anti-CD22 IgG2a raised against Raji Burkitt lymphoma cells. Murine sequences comprise 5–10% of the molecule, with the remainder being human framework sequences, which greatly reduces the potential for immunogenicity.
Reactivity
Human
Specificity
This antibody is specific for the 3rd Ig-like domain of human CD22 (epitope B), a cell surface glycoprotein present on mature B-cells and on many types of malignant B-cells.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG, Kappa
Recommend Usage
Flow cytometry
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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Applications
Flow Cytometry
Flow-cytometry were stained with an isotype control at a concentration of 1 ug/mL for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer. -
Gene Info — CD22
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Interactome
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Pathway
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Disease
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