Products

MYL6 (phospho S19/20) polyclonal antibody (Cat # PAB9961) was used at a 1 : 5000 dilution to detect myosin light chain by Western blot.
Either 13 or 20 uL of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock-treated or CLA-treated, as indicated. After washing, a 1 : 5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amersham's substrate system.
Other detection methods will yield similar results.
Data courtesy of the Alliance for Cellular Signaling (http://www.signaling-gateway.org).

MYL6 (phospho S19/20) polyclonal antibody (Cat # PAB9961) was used at a 1 : 1000 dilution to detect myosin light chain by Western blot on NIH/3T3 cell lysates.
A standard urea/glycerol gel without SDS was used to separate phospho forms of regulatory light chain according to mass to charge ratios.
In Panel A, reactivity of MYL6 (phospho S19/20) polyclonal antibody (Cat # PAB9961) is shown.
In Panel B, reactivity of commercially available pan reactive antibody that detects both unphosphorylated and phosphorylated forms of regulatory light chain is shown.The phosphospecific antibody detects both monophosphorylated (pSer20 Mono-P-RLC) and diphosphorylated (pThr19-pSer20 Di-P-RLC) regulatory light chain.
Personal communication. J. Stull. UT Southwestern Medical Center.

Immunohistochemical staining with MYL6 (phospho S19/20) polyclonal antibody (Cat # PAB9961) was used at 2.5 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows strong staining of both vascular and myometrial smooth muscle cells of the uterus.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.