AGAP2 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot using AGAP2 polyclonal antibody (Cat # PAB9929) shows detection of AGAP2 recombinant protein in transfected HEK293 cell lysates.
No specific band staining is noted in mocktransfected cell lysates (empty vector).
The membrane was probed with the primary antibody diluted to 1 : 10,000.
Personal Communication, Paul Randazzo, CCR-NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant AGAP2.
Immunogen
Recombinant protein corresponding to amino acids 1-836 of human AGAP2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Lyophilized
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:40000-1:200000)
Western Blot (1:5000-1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot using AGAP2 polyclonal antibody (Cat # PAB9929) shows detection of AGAP2 recombinant protein in transfected HEK293 cell lysates.
No specific band staining is noted in mocktransfected cell lysates (empty vector).
The membrane was probed with the primary antibody diluted to 1 : 10,000.
Personal Communication, Paul Randazzo, CCR-NCI, Bethesda, MD.Enzyme-linked Immunoabsorbent Assay
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Gene Info — AGAP2
Entrez GeneID
116986Protein Accession#
NP_055585;Q99490Gene Name
AGAP2
Gene Alias
CENTG1, FLJ16430, GGAP2, KIAA0167, PIKE
Gene Description
ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
Omim ID
605476Gene Ontology
HyperlinkGene Summary
O
Other Designations
Arf GAP with GTP-binding protein-like, ANK repeat and PH domains 2|GTP-binding and GTPase activating protein 2|centaurin, gamma 1|phosphoinositide 3-kinase enhancer
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Pathway
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Disease
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Publication Reference
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The Arf GAPs AGAP1 and AGAP2 distinguish between the adaptor protein complexes AP-1 and AP-3.
Nie Z, Fei J, Premont RT, Randazzo PA.
Journal of Cell Science 2005 Aug; 118(Pt 15):3555.
Application：WB, Human, A-549, AGS, HepG2, Mel14, SKOV3, SUM149, U87, VW373 cells.
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PIKE-A is amplified in human cancers and prevents apoptosis by up-regulating Akt.
Ahn JY, Hu Y, Kroll TG, Allard P, Ye K.
PNAS 2004 May; 101(18):6993.
Application：WB, Human, CRL-2061, LN229, LN-Z308, NGP127, SF188, TP366, U87MG cells.
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GGAPs, a new family of bifunctional GTP-binding and GTPase-activating proteins.
Xia C, Ma W, Stafford LJ, Liu C, Gong L, Martin JF, Liu M.
Molecular and Cellular Biology 2003 Apr; 23(7):2476.
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The Arf GAPs AGAP1 and AGAP2 distinguish between the adaptor protein complexes AP-1 and AP-3.
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