Cdc2 (phospho Y15) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot of human T-47D cells showing specific immunolabeling of the ~34k Cdc2 phosphorylated at Tyr15 (Control, lane 1). Treatment with EGF (30 ng per ml for 30 min) caused dephosphorylation of the Tyr15 on cdc2 (Lane 2). The phosphospecificity of this labeling is also shown in the third and fourth lanes (lambda-phosphatase: lambda-Ptase). These blots are identical to the control except that they were incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Phospho-Cdc2 Y15. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Cdc2.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Y15 of rat Cdc2.
Host
Rabbit
Theoretical MW (kDa)
34
Reactivity
Clawed frog, Human, Mouse, Rat, Zebra fish
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot of human T-47D cells showing specific immunolabeling of the ~34k Cdc2 phosphorylated at Tyr15 (Control, lane 1). Treatment with EGF (30 ng per ml for 30 min) caused dephosphorylation of the Tyr15 on cdc2 (Lane 2). The phosphospecificity of this labeling is also shown in the third and fourth lanes (lambda-phosphatase: lambda-Ptase). These blots are identical to the control except that they were incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Phospho-Cdc2 Y15. The immunolabeling is completely eliminated by treatment with lambda-Ptase. -
Gene Info — Cdc2
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Publication Reference
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Critical reanalysis of the methods that discriminate the activity of CDK2 from CDK1.
Sakurikar N, Eastman A.
Cell Cycle (Georgetown, Tex.) 2016 Mar; 15(9):1184.
Application：Cited references do not use this antibody.
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The E2F-Cdc2 cell-cycle pathway specifically mediates activity deprivation-induced apoptosis of postmitotic neurons.
Konishi Y, Bonni A.
The Journal of Neuroscience 2003 Mar; 23(5):1649.
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Suppression of Cdc2 dephosphorylation at the tyrosine 15 residue during nitrosourea-induced G2M phase arrest in glioblastoma cell lines.
Nakamizo A, Inamura T, Inoha S, Amano T, Ochi H, Ikezaki K, Fukui M.
Journal of Neuro-oncology 2002 Aug; 59(1):7.
Application：WB-Ce, Human, U373MG, U251MG cells.
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Cdc2 phosphorylation of nucleolin demarcates mitotic stages and Alzheimer's disease pathology.
Dranovsky A, Vincent I, Gregori L, Schwarzman A, Colflesh D, Enghild J, Strittmatter W, Davies P, Goldgaber D.
Neurobiology of Aging 2001 Jul; 22(4):517.
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Critical reanalysis of the methods that discriminate the activity of CDK2 from CDK1.
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