Gja1 (phospho S368) polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Tissue lysate)
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~43k Gja1 phosphorylated at Ser368 (Control, lane 1). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase, lane 2). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Gja1 (phospho S368) polyclonal antibody (Cat # PAB9652). The immunolabeling of Gja1 is completely eliminated by treatment with lambda-Ptase.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Gja1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S368 of rat Gja1.
Host
Rabbit
Theoretical MW (kDa)
43
Reactivity
Bovine, Chicken, Dog, Guinea pig, Human, Mouse, Rat, Zebra fish
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~43k Gja1 phosphorylated at Ser368 (Control, lane 1). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase, lane 2). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Gja1 (phospho S368) polyclonal antibody (Cat # PAB9652). The immunolabeling of Gja1 is completely eliminated by treatment with lambda-Ptase. -
Gene Info — Gja1
-
Publication Reference
-
Preserving protein profiles in tissue samples: Differing outcomes with and without heat stabilization.
Ahmed MM, Gardiner KJ.
Journal of Neuroscience Methods 2011 Mar; 196(1):99.
Application：WB, Mouse, Brain.
-
Regulation of purified and reconstituted connexin 43 hemichannels by protein kinase C-mediated phosphorylation of Serine 368.
Bao X, Reuss L, Altenberg GA.
The Journal of Biological Chemistry 2004 May; 279(19):20058.
-
Mechanism of regulation of the gap junction protein connexin 43 by protein kinase C-mediated phosphorylation.
Bao X, Altenberg GA, Reuss L.
American Journal of Physiology. Cell Physiology 2004 Mar; 286(3):C647.
-
Identification and functional analysis of novel phosphorylation sites in Cx43 in rat primary granulosa cells.
Yogo K, Ogawa T, Akiyama M, Ishida N, Takeya T.
FEBS Letters 2002 Nov; 531(2):132.
-
Preserving protein profiles in tissue samples: Differing outcomes with and without heat stabilization.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com