Th (phospho S31) polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot of PC-12 cells incubated in the absence (Control) and presence of okadaic acid (OA, 1 uM for 60 min) showing specific immunolabeling of the ~60k TH phosphorylated at Ser31.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Th.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S31 of rat Th.
Host
Rabbit
Theoretical MW (kDa)
60
Reactivity
Human, Mouse, Primates, Rat
Specificity
Specific for the 60 kDa tyrosine hydroxylase protein phosphorylated at Ser31 in lysates of PC-12 cells stimulated by Okadaic acid.
Form
Liquid
Purification
Affinity purification
Recommend Usage
Immunofluorescence (1:1000)
Immunohistochemistry (1:1000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 100 ug per mL BSA).
Storage Instruction
Store at -20°C for short term. For long term storage, store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot of PC-12 cells incubated in the absence (Control) and presence of okadaic acid (OA, 1 uM for 60 min) showing specific immunolabeling of the ~60k TH phosphorylated at Ser31.Immunohistochemistry (Frozen sections)
Immunofluorescence
-
Gene Info — Th
-
Publication Reference
-
Dexmedetomidine Ameliorated Cognitive Dysfunction Induced by Intestinal Ischemia Reperfusion in Mice with Possible Relation to the Anti-inflammatory Effect Through the Locus Coeruleus Norepinephrine System.
Gang Li, Jun Zhou, Jicheng Wei, Bin Liu.
Neurochemical Research 2022 Nov; 47(11):3440.
Application：IF, Mouse, Brain tissue.
-
Tyrosine hydroxylase phosphorylation: regulation and consequences.
Dunkley PR, Bobrovskaya L, Graham ME, von Nagy-Felsobuki EI, Dickson PW.
Journal of Neurochemistry 2004 Dec; 91(5):1025.
Application：WB-Ce, Bovine, Bovine adrenal chromaffin cells.
-
Acute administration of cocaine regulates the phosphorylation of serine-19, -31 and -40 in tyrosine hydroxylase.
Jedynak JP, Ali SF, Haycock JW, Hope BT.
Journal of Neurochemistry 2002 Jul; 82(2):382.
Application：WB-Ti, Rat, Rat caudate samples.
-
Striatal dopaminergic and serotonergic markers in human heroin users.
Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW.
Neuropsychopharmacology: Official Publication of the American College of Neuropsychopharmacology 2001 May; 24(5):561.
-
Dexmedetomidine Ameliorated Cognitive Dysfunction Induced by Intestinal Ischemia Reperfusion in Mice with Possible Relation to the Anti-inflammatory Effect Through the Locus Coeruleus Norepinephrine System.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com