Nsf polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot of rat caudate lysate showing specific labeling of the ~75k Nsf protein. Using Nsf polyclonal antibody (Cat # PAB9620).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Nsf.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus rat Nsf.
Host
Rabbit
Theoretical MW (kDa)
75
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat caudate lysate showing specific labeling of the ~75k Nsf protein. Using Nsf polyclonal antibody (Cat # PAB9620). -
Gene Info — Nsf
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Publication Reference
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Multiple binding proteins suggest diverse functions for the N-ethylmaleimide sensitive factor.
Whiteheart SW, Matveeva EA.
Journal of Structural Biology 2004 Apr; 146(1-2):32.
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Phosphorylation of the N-ethylmaleimide-sensitive factor is associated with depolarization-dependent neurotransmitter release from synaptosomes.
Matveeva EA, Whiteheart SW, Vanaman TC, Slevin JT.
The Journal of Biological Chemistry 2001 Apr; 276(15):12174.
Application：IP, Recombinant protein.
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Regulation of neurotransmitter release kinetics by NSF.
Schweizer FE, Dresbach T, DeBello WM, O'Connor V, Augustine GJ, Betz H.
Science 1998 Feb; 279(5354):1203.
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Multiple binding proteins suggest diverse functions for the N-ethylmaleimide sensitive factor.
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