MAP4K1 (phospho S171) polyclonal antibody
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More Files
- More Functions
Dot Blot (Peptide)
Dot blot analysis of MAP4K1 (phospho S171) polyclonal antibody (Cat # PAB8125) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAP4K1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S171 of human MAP4K1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Peptide affinity purification
Recommend Usage
ELISA (1:1000)
Dot Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot blot analysis of MAP4K1 (phospho S171) polyclonal antibody (Cat # PAB8125) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL. -
Gene Info — MAP4K1
Entrez GeneID
11184Protein Accession#
NP_009112;Q92918Gene Name
MAP4K1
Gene Alias
HPK1
Gene Description
mitogen-activated protein kinase kinase kinase kinase 1
Omim ID
601983Gene Ontology
HyperlinkOther Designations
hematopoietic progenitor kinase 1
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Interactome
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Pathway
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Publication Reference
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Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry.
Wissing J, Jänsch L, Nimtz M, Dieterich G, Hornberger R, Kéri G, Wehland J, Daub H.
Molecular & Cellular Proteomics 2007 Mar; 6(3):537.
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Large-scale characterization of HeLa cell nuclear phosphoproteins.
Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villen J, Li J, Cohn MA, Cantley LC, Gygi SP.
PNAS 2004 Aug; 101(33):12130.
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Human HPK1, a novel human hematopoietic progenitor kinase that activates the JNK/SAPK kinase cascade.
Hu MC, Qiu WR, Wang X, Meyer CF, Tan TH.
Genes & Development 1996 Sep; 10(18):2251.
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Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry.
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