PDPK1 (phospho Y9) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot of A-431 cells untreated (lanes 1) or treated with pervanadate (lanes 2, 3 & 4). Blots were probed with PDPK1 (phospho Y9) polyclonal antibody (Cat # PAB7926). The latter was used in the presence of no peptide (lane 2), phospho-PDK1 (Tyr-9) peptide (lane 3), or an unrelated phosphotyrosine peptide (lane 4).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of PDPK1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Y9 of human PDPK1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This sequence is highly conserved in rat and mouse PDK1.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot of A-431 cells untreated (lanes 1) or treated with pervanadate (lanes 2, 3 & 4). Blots were probed with PDPK1 (phospho Y9) polyclonal antibody (Cat # PAB7926). The latter was used in the presence of no peptide (lane 2), phospho-PDK1 (Tyr-9) peptide (lane 3), or an unrelated phosphotyrosine peptide (lane 4).Enzyme-linked Immunoabsorbent Assay
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Gene Info — PDPK1
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Interactome
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Pathway
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Disease
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Publication Reference
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Nox4 NAD(P)H oxidase mediates Src-dependent tyrosine phosphorylation of PDK-1 in response to angiotensin II: role in mesangial cell hypertrophy and fibronectin expression.
Block K, Eid A, Griendling KK, Lee DY, Wittrant Y, Gorin Y.
The Journal of Biological Chemistry 2008 Aug; 283(35):24061.
Application：WB, Rat, Rat glomerular MCs.
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Phosphoinositide-dependent phosphorylation of PDK1 regulates nuclear translocation.
Scheid MP, Parsons M, Woodgett JR.
Molecular and Cellular Biology 2005 Mar; 25(6):2347.
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Pyk2- and Src-dependent tyrosine phosphorylation of PDK1 regulates focal adhesions.
Taniyama Y, Weber DS, Rocic P, Hilenski L, Akers ML, Park J, Hemmings BA, Alexander RW, Griendling KK.
Molecular and Cellular Biology 2003 Nov; 23(22):8019.
Application：WB-Tr, Human, HEK 293 cells.
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Nox4 NAD(P)H oxidase mediates Src-dependent tyrosine phosphorylation of PDK-1 in response to angiotensin II: role in mesangial cell hypertrophy and fibronectin expression.
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