PDPK1 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PDPK1.
Immunogen
A synthetic peptide corresponding to N-terminus of human PDPK1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This sequence is highly conserved in rat and mouse PDK1.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot of A-431 cells untreated (lane 1) or treated with pervanadate (lane 2). Blots were probed with PDPK1 polyclonal antibody (Cat # PAB7885) .Enzyme-linked Immunoabsorbent Assay
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Gene Info — PDPK1
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Interactome
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Pathway
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Disease
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Publication Reference
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Phosphoinositide-dependent phosphorylation of PDK1 regulates nuclear translocation.
Scheid MP, Parsons M, Woodgett JR.
Molecular and Cellular Biology 2005 Mar; 25(6):2347.
Application:IF, WB, Human, MCF-7 cells.
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Pyk2- and Src-dependent tyrosine phosphorylation of PDK1 regulates focal adhesions.
Taniyama Y, Weber DS, Rocic P, Hilenski L, Akers ML, Park J, Hemmings BA, Alexander RW, Griendling KK.
Molecular and Cellular Biology 2003 Nov; 23(22):8019.
Application:IP-WB, WB-Tr, Human, Rat, Mouse, VSMCs, HEK 293, CHO/AT1 cells.
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Identification of tyrosine phosphorylation sites on 3-phosphoinositide-dependent protein kinase-1 and their role in regulating kinase activity.
Park J, Hill MM, Hess D, Brazil DP, Hofsteenge J, Hemmings BA.
The Journal of Biological Chemistry 2001 Oct; 276(40):37459.
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Phosphoinositide-dependent phosphorylation of PDK1 regulates nuclear translocation.
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