PNPLA3 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
PNPLA3 polyclonal antibody (Cat # PAB7591)(0.05 ug/mL) staining of mouse heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
PNPLA3 polyclonal antibody (Cat # PAB7591)(3.8 ug/mL) staining of paraffin embedded human skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PNPLA3.
Immunogen
A synthetic peptide corresponding to internal region of human PNPLA3.
Sequence
EHDICPKVKSTN
Host
Goat
Theoretical MW (kDa)
42.6
Reactivity
Human, Mouse
Specificity
This antibody is raised against a peptide derived from the mouse protein sequence and this peptide differs from human only in position 2.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:128000)
Western Blot (0.05-0.2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)(4-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PNPLA3 polyclonal antibody (Cat # PAB7591)(0.05 ug/mL) staining of mouse heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
PNPLA3 polyclonal antibody (Cat # PAB7591)(3.8 ug/mL) staining of paraffin embedded human skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PNPLA3
Entrez GeneID
80339Protein Accession#
NP_473429.2Gene Name
PNPLA3
Gene Alias
ADPN, C22orf20, iPLA(2)epsilon
Gene Description
patatin-like phospholipase domain containing 3
Omim ID
609567Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a triacylglycerol lipase that mediates triacylglycerol hydrolysis in adipocytes. The encoded protein, which appears to be membrane bound, may be involved in the balance of energy usage/storage in adipocytes. [provided by RefSeq
Other Designations
OTTHUMP00000028602|adiponutrin
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Interactome
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Pathway
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Disease
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