USP28 polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
USP28 polyclonal antibody (Cat # PAB7482) (3.8 ug/mL) staining of paraffin embedded human testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of USP28.
Immunogen
A synthetic peptide corresponding to internal region of human USP28.
Sequence
C-SVELKHYIQEDN
Host
Goat
Theoretical MW (kDa)
122
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5-3.8 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
USP28 polyclonal antibody (Cat # PAB7482) (3.8 ug/mL) staining of paraffin embedded human testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — USP28
Entrez GeneID
57646Protein Accession#
NP_065937.1Gene Name
USP28
Gene Alias
KIAA1515
Gene Description
ubiquitin specific peptidase 28
Omim ID
610748Gene Ontology
HyperlinkGene Summary
The ubiquitin-dependent protein degradation pathway is essential for proteolysis of intracellular proteins and peptides. Enzymes that remove ubiquitin from ubiquitin-conjugated peptides, like USP28, affect the fate and degradation of intracellular proteins and are essential for maintenance of cell-free ubiquitin pools (Valero et al., 2001).[supplied by OMIM
Other Designations
ubiquitin specific protease 28
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Interactome
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Publication Reference
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Fbw7 and Usp28 regulate myc protein stability in response to DNA damage.
Popov N, Herold S, Llamazares M, Schulein C, Eilers M.
Cell Cycle 2007 Oct; 6(19):2327.
Application：WB-Tr, Human, HeLa cells.
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Fbw7 and Usp28 regulate myc protein stability in response to DNA damage.
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