PENK polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
PENK polyclonal antibody (Cat # PAB7411) (0.3 ug/mL) staining of human adrenal gland lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Frozen sections)
PENK polyclonal antibody (Cat # PAB7411) (0.3 ug/mL) staining of PFA-perfused cryosection of human hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80°C for 30 min, Cy3-staining.
Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PENK.
Immunogen
A synthetic peptide corresponding to internal region of human PENK.
Sequence
C-RSHHQDGSDNEE
Host
Goat
Theoretical MW (kDa)
30.8
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (1-3 ug/mL)
Immunohistochemistry (Frozen sections) (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PENK polyclonal antibody (Cat # PAB7411) (0.3 ug/mL) staining of human adrenal gland lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Frozen sections)
PENK polyclonal antibody (Cat # PAB7411) (0.3 ug/mL) staining of PFA-perfused cryosection of human hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80°C for 30 min, Cy3-staining.
Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PENK
Entrez GeneID
5179Protein Accession#
NP_006202.1Gene Name
PENK
Gene Alias
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Gene Description
proenkephalin
Omim ID
131330Gene Ontology
HyperlinkOther Designations
enkephalin A|preproenkephalin
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Interactome
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Disease
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Publication Reference
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Food-associated cues alter forebrain functional connectivity as assessed with immediate early gene and proenkephalin expression.
Schiltz CA, Bremer QZ, Landry CF, Kelley AE.
BMC Biochemistry 2007 Apr; 5:16.
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Food-associated cues alter forebrain functional connectivity as assessed with immediate early gene and proenkephalin expression.
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