GABPB2 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
GABPB2 polyclonal antibody (Cat # PAB7286) (0.5 ug/mL) staining of HeLa cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of GABPB2.
Immunogen
A synthetic peptide corresponding to human GABPB2.
Sequence
C-SSENSSKATDETG
Host
Goat
Theoretical MW (kDa)
42.5, 38.1
Reactivity
Human
Specificity
This antibody is expected to recognize isoform beta 1 (NP_005245.2) and isoform gamma 1 (NP_0020.32.2).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.5-1.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
GABPB2 polyclonal antibody (Cat # PAB7286) (0.5 ug/mL) staining of HeLa cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GABPB1
Entrez GeneID
2553Protein Accession#
NP_005245.2;NP_0020.32.2Gene Name
GABPB1
Gene Alias
BABPB2, E4TF1, E4TF1-47, E4TF1-53, E4TF1B, GABPB, GABPB2, NRF2B1, NRF2B2
Gene Description
GA binding protein transcription factor, beta subunit 1
Omim ID
600610Gene Ontology
HyperlinkGene Summary
This gene encodes the GA-binding protein transcription factor, beta subunit. This protein forms a tetrameric complex with the alpha subunit, and stimulates transcription of target genes. The encoded protein may be involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. The crystal structure of a similar protein in mouse has been resolved as a ternary protein complex. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
Other Designations
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Interactome
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Publication Reference
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Identification and expression analysis of alternative transcripts of the mouse GA-binding protein (Gabp) subunits alpha and beta1.
O'Leary DA, Koleski D, Kola I, Hertzog PJ, Ristevski S.
Gene 2005 Jan; 344:79.
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Identification and expression analysis of alternative transcripts of the mouse GA-binding protein (Gabp) subunits alpha and beta1.
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