ASF1A polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ASF1A.
Immunogen
A synthetic peptide corresponding to human ASF1A.
Sequence
C-EDNTEKLEDAE
Host
Goat
Theoretical MW (kDa)
23
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:1000)
Western Blot (0.01-0.03 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
ASF1A polyclonal antibody (Cat # PAB7280) (0.01 ug/mL) staining of Jurkat cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ASF1A
Entrez GeneID
25842Protein Accession#
NP_054753.1Gene Name
ASF1A
Gene Alias
CGI-98, CIA, DKFZp547E2110, HSPC146
Gene Description
ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
Omim ID
609189Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. [provided by RefSeq
Other Designations
ASF1 anti-silencing function 1 homolog A|CCG1-interacting factor A|anti-silencing function 1A
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Interactome
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Publication Reference
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Asf1 is required for viability and chromatin assembly during DNA replication in vertebrate cells.
Sanematsu F, Takami Y, Barman HK, Fukagawa T, Ono T, Shibahara K, Nakayama T.
The Journal of Biological Chemistry 2006 May; 281(19):13817.
Application:WB-Ce, Chicken, DT40 cells.
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Asf1 is required for viability and chromatin assembly during DNA replication in vertebrate cells.
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