PSME1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
PSME1 polyclonal antibody (Cat # PAB7267) (0.3 ug/mL) staining of human peripheral blood mononucleocyte lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PSME1.
Immunogen
A synthetic peptide corresponding to human PSME1.
Sequence
C-KNFEKLKKPRGETK
Host
Goat
Theoretical MW (kDa)
28.7
Reactivity
Human
Specificity
This antibody is expected to recognize isoform 1 (NP_006254.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
PSME1 polyclonal antibody (Cat # PAB7267) (0.3 ug/mL) staining of human peripheral blood mononucleocyte lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PSME1
Entrez GeneID
5720Protein Accession#
NP_006254.1Gene Name
PSME1
Gene Alias
IFI5111, MGC8628, PA28A, PA28alpha, REGalpha
Gene Description
proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
Omim ID
600654Gene Ontology
HyperlinkGene Summary
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Two transcripts encoding different isoforms have been identified. [provided by RefSeq
Other Designations
11S regulator complex alpha subunit|29-kD MCP activator subunit|activator of multicatalytic protease subunit 1|interferon gamma up-regulated I-5111 protein|interferon-gamma IEF SSP 5111|interferon-gamma-inducible protein 5111|proteasome activator subunit
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Interactome
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Pathway
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Publication Reference
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Anti-20S proteasome autoantibodies inhibit proteasome stimulation by proteasome activator PA28.
Brychcy M, Kuckelkorn U, Hausdorf G, Egerer K, Kloetzel PM, Burmester GR, Feist E.
Arthritis & Rheumatism 2006 Jul; 54(7):2175.
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Anti-20S proteasome autoantibodies inhibit proteasome stimulation by proteasome activator PA28.
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