UCP2 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of UCP2.
Immunogen
A synthetic peptide corresponding to internal region of human UCP2.
Sequence
C-DSVKQFYTKGSEH
Host
Goat
Theoretical MW (kDa)
33.2
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:4000)
Western Blot (0.2-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
UCP2 polyclonal antibody (Cat # PAB7242)(0.2 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — UCP2
Entrez GeneID
7351Protein Accession#
NP_003346.2Gene Name
UCP2
Gene Alias
BMIQ4, SLC25A8, UCPH
Gene Description
uncoupling protein 2 (mitochondrial, proton carrier)
Gene Ontology
HyperlinkGene Summary
Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed in many tissues, with the greatest expression in skeletal muscle. It is thought to play a role in nonshivering thermogenesis, obesity and diabetes. Chromosomal order is 5'-UCP3-UCP2-3'. [provided by RefSeq
Other Designations
uncoupling protein 2|uncoupling protein-2
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Interactome
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Disease
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Publication Reference
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Mutant p53 blocks SESN1/AMPK/PGC-1α/UCP2 axis increasing mitochondrial O2-· production in cancer cells.
Cordani M, Butera G, Dando I, Torrens-Mas M, Butturini E, Pacchiana R, Oppici E, Cavallini C, Gasperini S, Tamassia N, Nadal-Serrano M, Coan M, Rossi D, Gaidano G, Caraglia M, Mariotto S, Spizzo R, Roca P, Oliver J, Scupoli MT, Donadelli M.
British Journal of Cancer 2018 Oct; 119(8):994.
Application:WB-Tr, Human, A-375, AsPC1, MeWo cells.
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UCP2 inhibition triggers ROS-dependent nuclear translocation of GAPDH and autophagic cell death in pancreatic adenocarcinoma cells.
Dando I, Fiorini C, Pozza ED, Padroni C, Costanzo C, Palmieri M, Donadelli M.
Biochimica et Biophysica Acta 2013 Mar; 1833(3):672.
Application:WB, Human, PaCa44 cells.
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Inhibition of UCP2 expression reverses diet-induced diabetes mellitus by effects on both insulin secretion and action.
Claudio T De Souza, Eliana P Araujo, Luiz F Stoppiglia, Jose R Pauli, Eduardo Ropelle, Silvana A Rocco, Rodrigo M Marin, Kleber G Franchini, Jose B Carvalheira, Mario J Saad, Antonio C Boschero, Everardo M Carneiro, Lício A Velloso.
FASEB Journal 2007 Apr; 21(4):1153.
Application:WB-Ti, Mouse, Mouse brain, Mouse heart , Mouse gastrocnemius muscle, Mouse pancreatic islets, Mouse spleen, Mouse white adipose tissues.
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Mutant p53 blocks SESN1/AMPK/PGC-1α/UCP2 axis increasing mitochondrial O2-· production in cancer cells.
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