GATA5 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
GATA5 polyclonal antibody (Cat # PAB7124) (0.3 ug/mL) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of GATA5.
Immunogen
A synthetic peptide corresponding to human GATA5.
Sequence
C-ELAARPGWAQTATAD
Host
Goat
Theoretical MW (kDa)
41.3
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
GATA5 polyclonal antibody (Cat # PAB7124) (0.3 ug/mL) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GATA5
Entrez GeneID
140628Protein Accession#
NP_536721.1Gene Name
GATA5
Gene Alias
bB379O24.1
Gene Description
GATA binding protein 5
Omim ID
611496Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transcription factor that contains two GATA-type zinc fingers. The encoded protein is known to bind to hepatocyte nuclear factor-1alpha (HNF-1alpha), and this interaction is essential for cooperative activation of the intestinal lactase-phlorizin hydrolase promoter. In other organisms, similar proteins may be involved in the establishment of cardiac smooth muscle cell diversity. [provided by RefSeq
Other Designations
GATA binding factor-5|GATA-binding protein 5|OTTHUMP00000031490|transcription factor GATA-5
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Interactome
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Publication Reference
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Promoter hypermethylation of multiple genes in sputum precedes lung cancer incidence in a high-risk cohort.
Belinsky SA, Liechty KC, Gentry FD, Wolf HJ, Rogers J, Vu K, Haney J, Kennedy TC, Hirsch FR, Miller Y, Franklin WA, Herman JG, Baylin SB, Bunn PA, Byers T.
Cancer Research 2006 Mar; 66(6):3338.
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Promoter hypermethylation of multiple genes in sputum precedes lung cancer incidence in a high-risk cohort.
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