GRIN2B polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
GRIN2B polyclonal antibody (Cat # PAB7054) (2 ug/mL) staining of rat brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunofluorescence
GRIN2B polyclonal antibody (Cat # PAB7054) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoskeleton and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of GRIN2B.
Immunogen
A synthetic peptide corresponding to human GRIN2B.
Sequence
C-KDAHEKDDFHHLS
Host
Goat
Theoretical MW (kDa)
166
Reactivity
Human, Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Immunofluorescence (10 ug/mL)
Western Blot (2 - 4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
GRIN2B polyclonal antibody (Cat # PAB7054) (2 ug/mL) staining of rat brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunofluorescence
GRIN2B polyclonal antibody (Cat # PAB7054) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoskeleton and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Enzyme-linked Immunoabsorbent Assay
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Gene Info — GRIN2B
Entrez GeneID
2904Protein Accession#
NP_000825.1Gene Name
GRIN2B
Gene Alias
MGC142178, MGC142180, NMDAR2B, NR2B, hNR3
Gene Description
glutamate receptor, ionotropic, N-methyl D-aspartate 2B
Omim ID
138252Gene Ontology
HyperlinkGene Summary
N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain. [provided by RefSeq
Other Designations
N-methyl-D-aspartate receptor subunit 2B|glutamate receptor subunit epsilon-2
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Interactome
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Pathway
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Disease
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Publication Reference
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NR2A and NR2B receptor gene variations modify age at onset in Huntington disease.
Arning L, Kraus PH, Valentin S, Saft C, Andrich J, Epplen JT.
Neurogenetics 2005 Feb; 6(1):25.
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NR2A and NR2B receptor gene variations modify age at onset in Huntington disease.
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