PIP polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
PIP polyclonal antibody (Cat # PAB7018) (0.3 ug/mL) staining of A-431 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
PIP polyclonal antibody (Cat # PAB7018) (2.5 ug/mL) staining of paraffin embedded human breast tissue. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PIP.
Immunogen
A synthetic peptide corresponding to human PIP.
Sequence
C-GANKAQDNTRK
Host
Goat
Theoretical MW (kDa)
16.6
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Western blot (0.3-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
PIP polyclonal antibody (Cat # PAB7018) (0.3 ug/mL) staining of A-431 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
PIP polyclonal antibody (Cat # PAB7018) (2.5 ug/mL) staining of paraffin embedded human breast tissue. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PIP
Entrez GeneID
5304Protein Accession#
NP_002643.1Gene Name
PIP
Gene Alias
GCDFP-15, GCDFP15, GPIP4
Gene Description
prolactin-induced protein
Omim ID
176720Gene Ontology
HyperlinkOther Designations
prolactin-inducible protein
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Interactome
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Disease
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Publication Reference
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Utility of immunohistochemistry in distinguishing primary adenocarcinomas from metastatic breast carcinomas in the gastrointestinal tract.
O'Connell FP, Wang HH, Odze RD.
Archives of Pathology & Laboratory Medicine 2005 Mar; 129(3):338.
Application：IHC-P, Human, Human gastrointestinal carcinomas, Human metastatic breast carcinomas.
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Utility of immunohistochemistry in distinguishing primary adenocarcinomas from metastatic breast carcinomas in the gastrointestinal tract.
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