USO1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
USO1 polyclonal antibody (Cat # PAB6897) (1 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of USO1.
Immunogen
A synthetic peptide corresponding to human USO1.
Sequence
C-DESEDPGKDLDH
Host
Goat
Theoretical MW (kDa)
108
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
USO1 polyclonal antibody (Cat # PAB6897) (1 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — USO1
Entrez GeneID
8615Protein Accession#
NP_003706.1Gene Name
USO1
Gene Alias
P115, TAP, VDP
Gene Description
USO1 homolog, vesicle docking protein (yeast)
Omim ID
603344Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Transport from the ER to the cis/medial Golgi compartments requires the action of this gene product, GM130 and giantin in a sequential manner. [provided by RefSeq
Other Designations
USO1 homolog, vesicle docking protein|transcytosis associated protein|transcytosis-associated protein|vesicle docking protein p115
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Interactome
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Publication Reference
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Golgin tethers define subpopulations of COPI vesicles.
Malsam J, Satoh A, Pelletier L, Warren G.
Science 2005 Feb; 307(5712):1095.
Application：WB-Ce, Cercopithecus aethiops, BSC-1 sells.
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Golgin tethers define subpopulations of COPI vesicles.
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