PIR polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
PIR polyclonal antibody (Cat # PAB6662) (0.3 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Transfected lysate)
HEK293 overexpressing PIR and probed with PIR polyclonal antibody (Cat # PAB6662) (mock transfection in first lane), tested by Origene.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PIR.
Immunogen
A synthetic peptide corresponding to human PIR.
Sequence
GSSKKVTLSVLSREC
Host
Goat
Theoretical MW (kDa)
32.1
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PIR polyclonal antibody (Cat # PAB6662) (0.3 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing PIR and probed with PIR polyclonal antibody (Cat # PAB6662) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PIR
Entrez GeneID
8544Protein Accession#
NP_0.03653Gene Name
PIR
Gene Alias
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Gene Description
pirin (iron-binding nuclear protein)
Omim ID
603329Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cupin superfamily. The encoded protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described. [provided by RefSeq
Other Designations
OTTHUMP00000022961|OTTHUMP00000022962|pirin
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Interactome
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Publication Reference
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Identification of pirin, a novel highly conserved nuclear protein.
Wendler WM, Kremmer E, Forster R, Winnacker EL.
The Journal of Biological Chemistry 1997 Mar; 272(13):8482.
Application：IF, IP, WB-Ce, Bovine, Dog, Human, Monkey, Mouse, Rat, 3T6, C6, COS-7, HeLa, K1, MDBK cells.
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Identification of pirin, a novel highly conserved nuclear protein.
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